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A Lateralized Odor Learning Model in Neonatal Rats for Dissecting Neural Circuitry Underpinning Memory Formation
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JoVE Journal Neuroscience
A Lateralized Odor Learning Model in Neonatal Rats for Dissecting Neural Circuitry Underpinning Memory Formation

A Lateralized Odor Learning Model in Neonatal Rats for Dissecting Neural Circuitry Underpinning Memory Formation

DOI:
10.3791/51808-v

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10:42 min

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August 18, 2014

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, , ,
1Division of Biomedical Sciences,Faculty of Medicine, Memorial University, 2Division of Medical Sciences,University of Victoria

Chapters

  • 00:05Title
  • 02:15Nose Plug Construction
  • 02:59Naris Occlusion Before Training
  • 04:23Scented Bedding Preparation
  • 05:19Odor Conditioning Paradigm
  • 06:26Lateralized Odor Preference Testing
  • 08:16Results: Naris Occlusion is an Effective Method for Isolating Odor Input and Producing Lateralized Odor Memory
  • 09:49Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol introduces lateralized early odor preference learning in rats using acute single naris occlusion. Lateralized learning permits the examination of behavioral outcomes and underpinning biological mechanisms within the same animals, reducing variance induced by between-animal designs. This protocol can be used to investigate molecular mechanisms underpinning early odor learning.

Tags

Keywords: Lateralized Odor LearningNeonatal RatsOlfactory CircuitryMemory FormationNaris OcclusionWithin-animal ComparisonOlfactory SystemOdor Memory

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