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C. elegans Positive Butanone Learning, Short-term, and Long-term Associative Memory Assays
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
C. elegans Positive Butanone Learning, Short-term, and Long-term Associative Memory Assays
DOI:

09:58 min

March 11, 2011

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Chapters

  • 00:05Title
  • 01:15Preparing and Preconditioning Worms
  • 02:47Short-term Associative Memory (Massed) Training
  • 04:24Long-term Associative Memory (Spaced) Training
  • 06:16Chemotaxis Assay
  • 08:21Short- and Long-term Associative Memory in Worms
  • 09:38Conclusion

Summary

Automatic Translation

Here we describe methods to test C. elegans associative learning and short- and long-term associative memory. These population assays employ the worms abilities to chemotax toward volatile odorants, and form positive associations upon pairing food with the chemoattractant butanone. Increasing the number of conditioning periods induces long-term memory.

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