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Aversive Associative Learning and Memory Formation by Pairing Two Chemicals in Caenorhabditis elegans
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Aversive Associative Learning and Memory Formation by Pairing Two Chemicals in Caenorhabditis elegans

Aversive Associative Learning and Memory Formation by Pairing Two Chemicals in Caenorhabditis elegans

DOI:
10.3791/64137-v

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07:17 min

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June 23, 2022

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, ,
1Information Processing Biology Unit,Okinawa Institute of Science and Technology Graduate University

Chapters

  • 00:05Introduction
  • 00:43Preparation of Synchronized Caenorhabditis elegans
  • 02:06Mass Training for Short-Term Associative Learning and Memory
  • 02:54Spaced Training for Long-term Associative Learning and Memory
  • 03:27Chemotaxis Assay
  • 05:47Results: Aversive Associative Learning and Memory Formation in Caenorhabditis elegans
  • 06:39Conclusion

Summary

Automatic Translation

Automatic Translation

We have previously developed protocols for Caenorhabditis elegans to form short- and long-term associative memories by massed and spaced training, respectively. Here, detailed protocols are described for the conditioning of C. elegans by pairing 1-propanol and hydrochloric acid as conditioned and unconditioned stimuli, respectively, to form aversive associative memory.

Tags

Keywords: Caenorhabditis ElegansLearningMemoryAversive Associative LearningChemical PairingNervous SystemBehavioral AnalysisNematode Growth MediumE. Coli OP50Gravid AnimalsAnimal Collector1-propanolHydrochloric AcidChemotaxis Assay

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