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JoVE Journal
Developmental Biology

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Identification of Critical Conditions for Immunostaining in the Pea Aphid Embryos
 

Identification of Critical Conditions for Immunostaining in the Pea Aphid Embryos: Increasing Tissue Permeability and Decreasing Background Staining

Article doi: 10.3791/53883
February 2nd, 2016

Summary February 2nd, 2016

A protocol of whole-mount immunostaining on aphid embryos is presented, in which critical conditions for decreasing background staining and increasing tissue permeability are addressed. These conditions are specifically developed for effective detection of protein expression in the embryonic tissues of aphids.

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