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Genetics
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Canalostomy 为成人和新生小鼠内耳局部药物传递的外科治疗方法
JoVE Journal
Genetics
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JoVE Journal
Genetics
Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice
Please note that all translations are automatically generated.
Click here for the English version.
Canalostomy 为成人和新生小鼠内耳局部药物传递的外科治疗方法
DOI:
10.3791/57351-v
•
09:34 min
•
May 25, 2018
•
Jing-Ying Guo
,
Lu He
,
Teng-Fei Qu
,
Yu-Ying Liu
,
Ke Liu
,
Guo-Peng Wang
,
Shu-Sheng Gong
1
Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital
,
Capital Medical University
,
2
Department of Otolaryngology-Head and Neck Surgery, Shanghai First People’s Hospital
,
Shanghai Jiao Tong University
Chapters
00:05
Title
00:56
Device Preparation
02:31
Canalostomy in Adult Mice
05:58
Canalostomy in Neonatal Mice
06:48
Results: Drug Delivery by Canalostomy Resulted in Widespread Distribution of the Reagent Throughout the Cochlea and Vestibular End-organs
08:13
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们描述了 canalostomy 程序, 允许当地药物传递到成年和新生小鼠的内耳通过半圆形运河, 对听力和前庭功能的最小损害。该方法可用于将病毒载体、药物和小分子接种到小鼠内耳内。
Tags
Canalostomy
Inner Ear Drug Delivery
Adult Mice
Neonatal Mice
Surgical Technique
Polyimide Tubing
Polyethylene Tubing
Injection Cannula
Fast Green Dye
AAV8-GFP
Streptomycin
Microinjection Pump
Postauricular Incision
Semicircular Canal
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