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Generation of Genome-wide Chromatin Conformation Capture Libraries from Tightly Staged Early Drosophila Embryos
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Genetics
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JoVE Journal Genetics
Generation of Genome-wide Chromatin Conformation Capture Libraries from Tightly Staged Early Drosophila Embryos

Generation of Genome-wide Chromatin Conformation Capture Libraries from Tightly Staged Early Drosophila Embryos

DOI:
10.3791/57001-v

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10:35 min

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October 03, 2018

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,
1Max Planck Institute for Molecular Biomedicine

Chapters

  • 00:04Title
  • 00:37Embryo Fixation
  • 01:48Embryo Sorting
  • 03:07In Situ Hi-C – Lysis
  • 04:57In Situ Hi-C – Digestion, Overhang Fill-in, and Ligation
  • 06:58DNA Extraction
  • 09:11Results: Analysis of Chromatin Conformation Capture Libraries from Drosophila Embryos
  • 09:58Conclusion

Summary

Automatic Translation

Automatic Translation

This work describes a protocol for the generation of high resolution in situ Hi-C libraries from tightly staged pre-gastrulation Drosophila melanogaster embryos.

Tags

Keywords: Chromatin Conformation CaptureChromatin ArchitectureGene ExpressionDevelopmentDrosophila EmbryosFormaldehyde FixationCell Cycle StagingEGFP PCNANuclear DensityNuclear CyclesFlash Freezing

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