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JoVE Journal
Developmental Biology

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Neurogenesis Using P19 Embryonal Carcinoma Cells
 

Neurogenesis Using P19 Embryonal Carcinoma Cells

Article DOI: 10.3791/58225-v 07:46 min April 27th, 2019
April 27th, 2019

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Summary

The P19 mouse embryonic carcinoma cell line (P19 cell line) is widely used for studying the molecular mechanism of neurogenesis with great simplification compared to in vivo analysis. Here, we present a protocol for retinoic acid-induced neurogenesis in the P19 cell line.

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Tags

Neurogenesis P19 Cell Line Neuron Differentiation User-friendly Method Maintenance Medium DMEM Glucose Fetal Bovine Serum Penicillin Streptomycin Cell Culture Confluent Cells PBS Trypsin-EDTA Centrifuge Pellet Cells Cell Counter
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