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An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery

Article DOI: 10.3791/54018-v • 07:43 min • August 23rd, 2016
August 23rd, 2016 •
1, 2, 3, 3, 4, 6, 3, 1, *2, *5
1Dept. of Biomedicine, Pharmacenter, University of Basel, 2Molecular Signalling and Gene Therapy, Narayana Nethralaya Foundation, Narayana Health City, 3Brain Ischemia and Regeneration, Department of Biomedicine, University Hospital Basel, 4Department of Neurosurgery, Klinikum Idar-Oberstein, 5Department of Neurosurgery and Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 6Department of Neurology, Laboratory of Molecular Neuro Oncology, University Hospital of Zurich
* These authors contributed equally

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Summary
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Epithelial to mesenchymal transition (EMT) allows cancers to become invasive. To investigate EMT, a neural stem cell (NSC)-based in vitro model devoid of serum and enzymes is described. This standardized system allows quantitative and qualitative assessment of cell migration, gene and protein expression. The model is suited for drug discovery.

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Enzyme-free Serum-free Neural Stem Cell Culture Model EMT Investigation Drug Discovery Migration Induction Compound Identification Cell Migration Invasion Non-migratory Cells Invasive Cells Neural Stem Cells Epithelial To Mesenchymal Transition High-grade Gliomas Embryonic Day 14.5 Rat Embryos Digit Formation Petri Dishes PBS Embryonic Hulls Expansion Medium
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