Neonatal Pial Surface Electroporation

Journal

Neuroscience

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Neonatal Pial Surface Electroporation

DOI:

10.3791/51319-v

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06:22 min

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May 07, 2014

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Rachelle Levy, Jessica Molina, Moise Danielpour*¹, Joshua J. Breunig*²

¹Department of Neurosurgery, Regenerative Medicine Institute,Cedars-Sinai Medical Center, ²Department of Biomedical Sciences, Regenerative Medicine Institute,Cedars-Sinai Medical Center

Chapters

00:05Title
01:21Preparation
02:36Pial Surface Plasmid Injection
03:38Electroporation
05:27Results: Pial Surface Expression
05:58Conclusion

Summary

Automatic Translation

The pial surface is a unique progenitor zone in the CNS that is receiving increasing attention. Herein, we detail a method for rapid genetic manipulation of this progenitor zone using a modified electroporation method. This procedure can be used for cellular and molecular investigations of cell lineages and signaling pathways involved in cell differentiation and to elucidate the fate and properties of daughter cells.

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Chapters

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