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Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
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Developmental Biology
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JoVE Journal Developmental Biology
Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

DOI:
10.3791/59690-v

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12:13 min

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July 11, 2019

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1Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration,Ministry of Education, 2Center of Neural Injury and Repair,Beijing Institute for Brain Disorders

Chapters

  • 00:04Title
  • 01:31Isolation of PBMNCs (Peripheral Blood Mononuclear Cells)
  • 03:00Expansion of MNCs
  • 04:12Reprogramming of PBMNCs to iNSCs (Induced Neural Stem Cells) by SeV (Sendai Virus) Infection
  • 07:20Establishment of Unilateral 6-Hyroxydopamine (6-OHDA)-Lesioned Parkinson s Disease (PD) Mouse Models
  • 09:15Results: Induced Neural Stem Cells Differentiation Toward Dopaminergic Neuron Precursors and Treatment of Parkinson s Disease Mouse Models
  • 11:00Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol presents the reprogramming of peripheral blood mononuclear cells to induce neural stem cells by Sendai virus infection, differentiation of iNSCs into dopaminergic neurons, transplantation of DA precursors into the unilaterally-lesioned Parkinson's disease mouse models, and evaluation of the safety and efficacy of iNSC-derived DA precursors for PD treatment.

Tags

Keywords: Induced Neural Stem CellsPeripheral Mononuclear CellsDopaminergic Neuron PrecursorsParkinson’s DiseaseAutologous Cell SourceProliferative CapacityRegion-specific Neural CellsNeurological DiseasesDensity Gradient MediumMononuclear CellsCell ExpansionCell FreezingCell Counting

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