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TChIP-Seq: Cell-Type-Specific Epigenome Profiling
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TChIP-Seq: Cell-Type-Specific Epigenome Profiling

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

DOI:
10.3791/58298-v

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07:28 min

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January 23, 2019

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, , ,
1RNA Biology Laboratory,RIKEN, 2RNA Systems Biochemistry Laboratory,RIKEN Cluster for Pioneering Research, 3Laboratory for Phyloinformatics,RIKEN Center for Biosystems Dynamics Research, 4RNA Biology Laboratory, Faculty of Pharmaceutical Sciences,Hokkaido University, 5Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences,University of Tokyo

Chapters

  • 00:04Title
  • 00:49Tissue Dissection
  • 01:25Cell Fixation
  • 04:45Chromatin Shearing
  • 05:33Results: Tandem Chromatin Immunoprecipitation Sequencing (tChIP-Seq) Validated
  • 06:53Conclusion

Summary

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Automatic Translation

We describe a step-by-step protocol for tandem chromatin immunoprecipitation sequencing (tChIP-Seq) that enables the analysis of cell-type-specific genome-wide histone modification.

Tags

Keywords: TChIP-SeqCell-type-specificEpigenome ProfilingChromatin ModificationsHistone ModificationsNeuron-specificCryogenic GrindingFormaldehyde FixationChromatin Isolation

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