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Immunology and Infection
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Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice
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Immunology and Infection
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JoVE Journal Immunology and Infection
Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice

Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice

DOI:
10.3791/58930-v

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08:52 min

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February 22, 2019

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, , ,
1Department of Microbial Infection & Immunity,The Ohio State University, 2Department of Microbiology,The Ohio State University

Chapters

  • 00:04Title
  • 00:31Immunization and Intranasal Infection
  • 01:58Tissue Harvest
  • 05:49Lung Processing
  • 07:04Results: Representative Host-Pathogen Response and Vaccine Efficacy Analyses
  • 07:56Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present an elegant protocol for in vivo evaluation of vaccine effectiveness and host immune responses. This protocol can be adapted for vaccine models that study viral, bacterial, or parasitic pathogens.

Tags

Keywords: Host-pathogen ResponseVaccine EfficacyMouse ModelImmunizationBacterial InfectionOrgan HarvestSpleenRPMIFetal Bovine Serum

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