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Single-overførselshastighed supplerende høj opløsning analytiske teknikker til kendetegner komplekse naturlig organisk materiale blandinger
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1Environmental Molecular Sciences Laboratory,Pacific Northwest National Laboratory, 2Department of Soil, Water and Environmental Science,University of Arizona, 3Department of Earth Ocean and Atmospheric Sciences,Florida State University, 4Bruker Daltonics Inc., 5Biological Sciences Division,Pacific Northwest National Laboratory
Chapters
- 00:04Title
- 01:11Sequential Extraction of Organic Matter from Soil, Sediments, or Peat
- 02:42FTICR-MS Analysis
- 04:55Chemical Derivatization for GC-MS
- 06:14Liquid State NMR Analysis and LC-MS Lipidomics Analysis
- 07:22Proteomics Analysis
- 07:37Results: Number of Enzymes Identified in Minnesota Peat at Three Different Depths
- 09:04Conclusion
Denne protokol beskriver et enkelt gennemløb for supplerende analytiske og omik teknikker kulminerede i en fuldt parret karakterisering af naturligt organisk materiale og mikrobielle proteomics i forskellige økosystemer. Denne fremgangsmåde tillader robust sammenligninger til identifikation af metaboliske reaktionsveje og transformationer vigtigt til at beskrive greenhouse gasproduktion og forudsige svar til miljøændringer.
