Name: Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level (Video) | JoVE Journal
Uploaded: 2019-04-19T13:30:00.000+00:00
Duration: 8 min 29 s
Description: Learn single-molecule quantification of protein labeling homogeneity using fluorescent counting techniques. This method reveals heterogeneity invisible to bulk measurements and enables ultrasensitive diagnostic assays.

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5 minutes

The authors thank Masae Ohno and Kazuya Nishimura for experimental assistance and advice. This work was supported by PRESTO (JPMJPR15F7), Japan Science and Technology Agency, Grants-in-aid for Young Scientists (A) (24687022), Challenging Exploratory Research (26650055), and Scientific Research on Innovative Areas (23115005), Japan Society for the Promotion of Science, and by grants from the Takeda Science Foundation and the Mochida Memorial Foundation for Medical and Pharmaceutical Research. S.L. acknowledges support from the RIKEN International Program Associate (IPA) program.

1. Cell preparation
<ol>
	<li>Cultivate HeLa cells in a 10 cm dish at 37 &#176;C under 5% CO2 in Dulbecco&#8217;s modified Eagle&#8217;s medium containing 10% fetal bovine serum.</li>
	<li>Collect exponentially growing cells once they have reached 70% confluency, following ATCC instructions17.
	<ol>
		<li>Rinse cells with 5 mL of 1x phosphate buffered saline (PBS) (pH 7.4). Remove PBS.</li>
		<li>Add 1 mL of 0.1% trypsin-ethylenediaminetetraacetic acid (EDTA). Incubate 5 min at 37 &#176;C.</li>
		<li>Monitor the progression of cell dissociation by microscopy.</li>
		<li>Once cells become round and are detached from the dish, add 4 mL of growth medium, and break cell agglomeration by pipetting.</li>
		<li>Count the number of cells using a cell counter.</li>
		<li>Centrifuge at 150 x g for 10 min in a centrifuge tube. Remove the medium and resuspend the cell pellet with 5 mL of 1x PBS (pH 7.4).</li>
		<li>Repeat step 1.2.6. Resuspend cells in 1x PBS (pH 7.4) to a final concentration of 106 cells/mL using the count number in step 1.2.5. 
		NOTE: The cell suspension can be aliquoted and stored at -80 &#176;C for several months. Repeating a freeze/thaw cycle should be avoided.</li>
	</ol>
	</li>
</ol>
2. Cell lysis and fluorescent labeling
<ol>
	<li>Make 10 mL of lysing buffer (0.1 M borate, 1% sodium dodecyl sulfate (SDS) and 1% Tween 20). Adjust to pH 12 using 2 M NaOH. 
	CAUTION: Concentrated NaOH is corrosive. Wear suitable gloves and glasses when preparing this solution. 
	NOTE: Lysing buffer can be stored for up to one week at room temperature.</li>
	<li>Mix 100 &#956;L of lysing buffer with 1 &#956;L of 1 M dithiothreitol (DTT) and 4 &#956;L of 50% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS).</li>
	<li>Mix 1 &#956;L of the cell culture (approximately 1,000 cells) with the mixed lysing buffer from step 2.2. Homogenize the solution by slowly pipetting to avoid bubbles. Incubate in the dark at room temperature for 5 min with gentle agitation. Re-homogenize the solution by pipetting slowly.</li>
	<li>Add 1 &#956;L of 2 &#956;g/mL Cy3 NHS-ester dye. Homogenize the solution by slowly pipetting to avoid bubbles. Incubate for 5 min in the dark at room temperature with gentle agitation. 
	NOTE: The high pH of this buffer causes neutralization of lysine residues in proteins, leading to a higher reactivity.</li>
	<li>Repeat the addition of 1 &#956;L of 2 &#956;g/mL Cy3 NHS-ester dye. Homogenize the solution by slowly pipetting to avoid bubbles. Incubate for 10 min in the dark at room temperature with gentle agitation. 
	NOTE: This repeated addition of NHS-ester dye can increase the labeling efficiency because some of the Cy3 NHS-ester dye is hydrolyzed and deactivated by the high pH of the reaction buffer.</li>
	<li>Adjust to pH 7.2 by adding 100 &#956;L of 0.8 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-NaOH pH 7.2 to quench the reaction. Homogenize the solution by slowly pipetting to avoid bubbles.</li>
	<li>Add 20 &#956;L of 19 mg/mL biotin-(polyethylene glycol (PEG))2-amine and 5 &#956;L of 20 mg/mL 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). Homogenize the solution by slowly pipetting to avoid bubbles. Incubate for 1 h in the dark at room temperature with gentle agitation. 
	NOTE: This biotinylation step is necessary to fix the protein molecules on a microscope coverslip at a fixed density and with no bias for protein species.</li>
	<li>Remove unreacted labeling reagents and concentrate the sample using an ultrafiltration column with 10 kDa cut-off, following the manufacturer&#39;s instructions. 
	NOTE: The protocol can be paused here. The sample can be stored at -80 &#176;C for several weeks.</li>
</ol>
3. Proteome separation and recovery
<ol>
	<li>Add 4x SDS sample buffer (200 mM Tris-HCl pH 6.8, 4% SDS, 4% glycerol and 0.4% bromophenol blue). 
	NOTE: To avoid dye damage, the protein sample should not be heated as done in standard SDS-PAGE, and should be kept at room temperature.</li>
	<li>Perform standard SDS-PAGE for the protein sample and a molecular ladder, using 20% acrylamide gel. Migrate the sample in the gel at a constant voltage (160 V) until the migration front just exits the gel.</li>
	<li>Image the gel to identify the location of the protein bands (Figure 3).</li>
	<li>Cut out the gel portions including protein fractions of interest using a sharp blade, based on the band locations. Fractioning at 16, 23, 55 and 120 kDa peaks, and at 19&#8211;25, 25&#8211;32, 32&#8211;42, 42&#8211;54, 54&#8211;69, 69&#8211;97, 97&#8211;136 and 136&#8211;226 kDa regions are shown in Figure 3.</li>
	<li>Extract proteins using a dialyzer by electroelution with a pore size of 6&#8211;8 kDa, following the manufacturer&#39;s instructions. 
	NOTE: The extracted sample can be stored at -80 &#176;C for several weeks.</li>
</ol>
4. Microscope coverslip preparation
<ol>
	<li>Prepare 200 &#956;L of avidin buffer (5 mM HEPES-NaOH pH 7.2, 10 ng/mL avidin and 2 mg/mL bovine serum albumin (BSA)) for each sample.</li>
	<li>Prepare coverslips of 22 mm x 22 mm and 0.15 mm thickness. Expose the coverslips to air plasma using a plasma cleaner for 1 min to clean and activate their surfaces.</li>
	<li>Coat the coverslips with avidin by spin-coating 200 &#956;L of avidin buffer using a spin coater for 5 seconds at 500 rpm, followed by 30 seconds at 1,000 rpm. Incubate the coverslips for 15 min at room temperature to let them dry.</li>
	<li>Prepare coverslips for the protein sample.
	<ol>
		<li>Dilute the protein sample (from 3.5) 100 times in 5 mM HEPES-NaOH pH 7.2.</li>
		<li>Place 100 &#956;L of the diluted sample on the surface at the center of the avidin-coated coverslip (from step 4.3).</li>
		<li>Incubate in the dark for 15 min at room temperature.</li>
	</ol>
	</li>
	<li>Prepare the positive control.
	<ol>
		<li>Place 100 &#956;L of 10 ng/mL purified fluorescent biotin in 5 mM HEPES-NaOH pH 7.2 at the center of the avidin coated coverslip (from step 4.3).</li>
		<li>Incubate in the dark for 15 min at room temperature. 
		NOTE: This positive control coverslip provides the density of avidin binding spots, which is used to calculate the LO.</li>
	</ol>
	</li>
	<li>Prepare the negative control.
	<ol>
		<li>Spin-coat the plasma cleaned coverslip with 200 &#956;L of 5 mM HEPES-NaOH and 2 mg/mL BSA (without avidin).</li>
		<li>Incubate for 15 min at room temperature to dry.</li>
		<li>Add 100 &#956;L of 10 ng/mL purified fluorescent biotin in 5 mM HEPES-NaOH pH 7.2.</li>
		<li>Incubate in the dark for 15 min at room temperature. 
		NOTE: This negative control coverslip provides the amount of non-specific binding of fluorescent biotin to the coverslip without avidin.</li>
	</ol>
	</li>
	<li>Rinse each coverslip with 200 &#956;L of distilled water by pipetting at the edges of the coverslip so as not to touch the middle of the coverslip with the tip. Repeat this wash three times. 
	NOTE: After aspirating water on the coverslip, water should be immediately placed back on the coverslip to prevent it from drying out completely.</li>
	<li>Expose air plasma to the same number of coverslips as in step 4.2.</li>
	<li>Place the cleaned coverslip on the sample-bound coverslip from step 4.7 to avoid drying.</li>
</ol>
5. Observation with single molecule fluorescence microscopy
<ol>
	<li>Start up a wide-field or evanescent field fluorescence microscope. 
	CAUTION: Direct or scattered laser radiation can cause eye or skin damage. Wear suitable protective goggles and shield the laser light path. 
	NOTE: For the microscope setup, please refer to our previous publication16. Briefly, a wide-field or evanescent field fluorescence microscope equipped with a high-power 488 nm laser excitation, a high numerical aperture objective lens and a high sensitivity camera can be used for this measurement. Further, for an automated measurement, a computer-controlled XY translational sample stage, mechanical shutters for laser excitations and an auto-focusing system are recommended to be installed.</li>
	<li>Set the coverslip on the microscope and find the focus.</li>
	<li>Perform a tile scan to obtain at least 100 images. 
	NOTE: The laser illumination should be controlled with mechanical shutters to be exposed to the sample only when recording images with the camera, to minimize photobleaching. If there are many samples, automated measurement using a computer program to control the microscope devices is recommended. Each image typically includes 100&#8211;500 single molecule spots when using a 60x objective lens.</li>
</ol>
6. Image analysis and extraction of information
<ol>
	<li>If the image has unevenness due to the laser illumination pattern, carry out image processing to correct it18.
	<ol>
		<li>Acquire a reference image by measuring with a coverslip sample consisting of uniformly-spread dyes using a camera setting the same as in step 5.3.</li>
		<li>Acquire an offset image by measuring with no laser excitation using the same camera setting.</li>
		<li>Subtract each pixel value in every sample image and the reference image with the value of the offset image.</li>
		<li>Divide each pixel value in the subtracted sample images by the value in the subtracted reference image.</li>
	</ol>
	</li>
	<li>Collect properly acquired images and remove the image background.
	<ol>
		<li>Remove images containing large fluorescent aggregates manually.</li>
		<li>Remove the image background by applying the rolling-ball algorithm19 with a ball radius of 50 pixels using ImageJ20.</li>
		<li>Sharpen images by subtracting blurred images using ImageJ (sharpen image function).</li>
	</ol>
	</li>
	<li>Find and analyze single molecule spots in images.
	<ol>
		<li>Identify single molecule spots with the Yen threshold algorithm21 using ImageJ.</li>
		<li>Filter spots with less than two pixels to exclude dark noise of the camera and spots more than 20 pixels to exclude aggregations of proteins using ImageJ (ROI manager).</li>
		<li>Count the number of spots in each image. Using the illumination corrected images (step 6.1.4), analyze total intensities within pixels for every spot using ImageJ.</li>
	</ol>
	</li>
	<li>Calculate the labeling occupancy (LO) using the following formula: 
	LO = (number of counts in the sample / number of counts in the positive control) x 100</li>
	<li>Calculate the average number of fluorescent dyes bound to a single protein molecule (n&#772;dye).
	<ol>
		<li>Analyze a histogram of intensities of every spot. 
		NOTE: The histogram usually has several peaks, and every peak represents a different number of dye molecules bound to a protein. The first, second and third peak in the histogram corresponds to 1, 2 and 3 molecules, respectively.</li>
		<li>Calculate the average intensity from the histogram. Also calculate the peak intensity of the first peak by applying a Gaussian fitting.</li>
		<li>Calculate n&#772;dye by dividing the average intensity with the peak intensity.</li>
	</ol>
	</li>
</ol>

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The authors declare the following competing financial interest(s): RIKEN has filed a patent application on these results with S.L. and Y.T. named as co-inventors.

This paper describes a protocol to quantify the labeling homogeneity of each labeled protein species in cells after separation with SDS-PAGE (step 3). The separation method can be substituted with other methods such as liquid chromatography or capillary electrophoresis, which allow separation and fractionation of cellular proteins with high resolution, while requiring special equipment23. The labeling method using NHS-ester in the current protocol can be replaced with other methods using maleimide...

Proteome analysis, which aims to quantify the entire set of protein molecules expressed in the cell, is a valuable approach in current biological and medicinal studies. This analysis commonly relies on mass spectrometry, which identifies protein species based on spectra generated through protein ionization1,2,3. An alternative method for proteome analysis is electrophoresis, including polyacrylamide gel electrophoresis (PAGE), capillary electrophoresis and two-dimensional (2D) gel electrophoresis. This method relies on non-specific fluorescent labeling of all the protein molecules in the analyzed cells, followed by electrophoretic separation and detection and quantification of each protein species. To achieve the required non-specific protein labeling, one strategy is to use fluorescent dyes that can bind to proteins via electrostatic and hydrophobic interactions, such as Coomassie Blue and Sypro-Ruby4,5,6. An alternative strategy is to use covalent labeling with dyes containing N-hydroxysuccinimide (NHS) ester or maleimide, which can covalently bind to proteins through common residues such as primary amines and thiols, respectively7,8.
Meanwhile, the sensitivity of fluorescence detection is ideal for analyzing low-abundance proteins and small numbers of cells. Single molecule fluorescence imaging is one of the most sensitive methods that allows the detection of individual fluorescent dyes and labeled proteins in vitro and in vivo9,10,11,12,13,14,15. Application of this imaging method to electrophoresis-based proteome analysis is expected to enable highly sensitive and quantitative assays by counting individual fluorescently-labeled proteins. However, it remains unclear whether labeling with fluorescent dyes is homogeneous enough across all the protein molecules, and how this homogeneity is affected by different protein species (Figure 1). Simple bulk solution measurements can be used to obtain a molar ratio of fluorescent dyes to proteins called &#8216;coupling efficiency&#8217;8 or &#8216;labeling efficiency&#8217;, but this property does not provide information on the homogeneity of the labeling among protein molecules.
Here, we describe the protocol for an assay to investigate the labeling homogeneity for all protein species in the cell (Figure 2)16. The two key steps of this assay are protein purification and imaging. In the first step, all the proteins in the cells are fluorescently labeled and biotinylated, then extracted separately using gel electrophoresis followed by electroelution. In the second step, fluorescence properties of individual protein molecules in the extracted samples are evaluated based on single molecule imaging. From this data, parameters important for the counting analysis, such as the percentage of proteins labeled with a least one dye, which we call labeling occupancy (LO)16, and the average number of fluorescent dyes bound to a single protein molecule (n&#772;dye), can be characterized. In the protocol, an optimized procedure for labeling the HeLa cell proteome with NHS ester-based Cyanine 3 (Cy3) dye is presented as an example, and can be modified with other labeling procedures according to desired research goals.

<table><tbody><tr><td>22x22x0.15 mm coverslip</td><td>VWR</td><td>470019-004</td><td></td></tr><tr><td>488 nm Argon laser</td><td>Coherent</td><td>Innova 70C</td><td></td></tr><tr><td>488 nm dichroic mirror</td><td>Semrock</td><td>FF495-Di03</td><td></td></tr><tr><td>488 nm emission filter</td><td>Semrock</td><td>FF02-520/28</td><td></td></tr><tr><td>560 nm dichroic filter</td><td>Semrock</td><td>Di02-R561</td><td></td></tr><tr><td>560 nm emission filter</td><td>Semrock</td><td>FF02-617/73</td><td></td></tr><tr><td>560 nm fiber laser</td><td>MBP Communications</td><td>F-04306-2</td><td></td></tr><tr><td>60x oil immersion lens</td><td>Olympus</td><td>PLAPON 60x</td><td></td></tr><tr><td>Avidin</td><td>Nacalai-tesque</td><td>03553-64</td><td></td></tr><tr><td>Biotin-PEG-amine</td><td>Thermo Scientific</td><td>21346</td><td></td></tr><tr><td>Biotinylated Alexa Fluor 488</td><td>Nanocs</td><td></td><td></td></tr><tr><td>Borate</td><td>Nacalai-tesque</td><td></td><td></td></tr><tr><td>BSA</td><td>Sigma-Aldrich</td><td>A9547</td><td></td></tr><tr><td>CHAPS</td><td>Dojindo</td><td>C008-10</td><td></td></tr><tr><td>Cy3 NHS-ester dye</td><td>GE Healthcare</td><td>PA13101</td><td></td></tr><tr><td>Dialyzer&amp;nbsp; - D-tube, 6-8 kDa</td><td>Merck Millipore</td><td>71507-M</td><td></td></tr><tr><td>DMEM</td><td>Sigma-Aldrich</td><td></td><td></td></tr><tr><td>DTT</td><td>Nacalai-tesque</td><td>14112-94</td><td></td></tr><tr><td>EDC</td><td>Nacalai-tesque</td><td></td><td></td></tr><tr><td>EMCCD camera</td><td>Andor</td><td>IiXon 897</td><td></td></tr><tr><td>Epi fluorescence microscope</td><td>Olympus</td><td>IX81</td><td></td></tr><tr><td>Gel viewer</td><td>GE Healthcare</td><td>ImageQuant LAS 4000</td><td></td></tr><tr><td>Penicillin, streptomycine and Amphoterecin mix</td><td>Gibco</td><td></td><td></td></tr><tr><td>Plasma cleaner</td><td>Diener Electronic</td><td></td><td></td></tr><tr><td>SDS</td><td>Wako</td><td>NC0792960</td><td></td></tr><tr><td>Size purification column 10K</td><td>Merck Millipore</td><td>UFC5010</td><td></td></tr><tr><td>Tween 20</td><td>Sigma-Aldrich</td><td>P9416</td><td></td></tr></tbody></table>

proteome-wide quantification of labeling homogeneity at the single molecule level

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Cell proteomes are often characterized using electrophoresis assays, where all species of proteins in the cells are non-specifically labeled with a fluorescent dye and are spotted by a photodetector following their separation. Single molecule fluorescence imaging can provide ultrasensitive protein detection with its ability for visualizing individual fluorescent molecules. However, the application of this powerful imaging method to electrophoresis assays is hampered by the lack of ways to characterize the homogeneity of fluorescent labeling of each protein species across the proteome. Here, we developed a method to evaluate the labeling homogeneity across the proteome based on a single molecule fluorescence imaging assay. In our measurement using a HeLa cell sample, the proportion of proteins labeled with at least one dye, which we termed &#8216;labeling occupancy&#8217; (LO), was determined to range from 50% to 90%, supporting the high potential of the application of single molecule imaging to sensitive and precise proteome analysis.

Figure 4 represents raw image data for different molecular weight fractions of proteins from HeLa cell lysate, as well as the positive and negative control. While both the protein sample and positive control exhibit 100–500 spots per image, the negative control displays none or a few spots, showing that the protocol sufficiently inhibits non-specific binding of dyes to the coverslip surface. Spot intensity histograms for the protein samples and the positive c...

Here, we present a protocol to assess the labeling homogeneity for each protein species in a complex protein sample at the single molecule level.

Cell proteomes are often characterized using electrophoresis assays, where all species of proteins in the cells are non-specifically labeled with a ...

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Research

JoVE Journal

Biochemistry

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Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

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"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

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Automated Sample Preparation for the Multiplexed Analysis of Single-Cell Histone Post-Translational Modifications (sc-hPTM2)

Learn single-molecule quantification of protein labeling homogeneity using fluorescent counting techniques. This method reveals heterogeneity invisible to bulk measurements and enables ultrasensitive diagnostic assays.

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

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