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Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Article DOI: 10.3791/59199-v • 08:29 min • April 19th, 2019
April 19th, 2019 •
1,2, 2, 1,3
1Laboratory for Cell Systems Control, RIKEN Center for Biosystems Dynamics Research, RIKEN, 2Laboratoire de Biomatériaux et Bioimagerie, INSERM, 3PRESTO, Japan Science and Technology Agency

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Summary
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Here, we present a protocol to assess the labeling homogeneity for each protein species in a complex protein sample at the single molecule level.

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Proteome-wide Quantification Labeling Homogeneity Single Molecule Level Protein Amount Liquid Sample Single-molecule Process Fluorescent Labeling Protein Populations Bulk Measurements Molar Ratios Heterogeneously Labeled Ultrasensitive Molecular Concentration Assays Diagnostic Methods Pathogenic Molecules Specimens Single Protein Species Analysis Fluorescent Antibodies Multiple Species Analysis Electrophoresis Chromatography Data Reproducibility Experimental Condition Visual Demonstration Stable And Reproducible Experimental Steps Spin-coating Washing Of Microscope Coverslips
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