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Chapters
- 00:04Title
- 00:53Adipose Tissue Collection and Antibody Incubation
- 05:17Image Acquisition and Analysis of Blood Vessel and Nerve Fiber Networks
- 09:37Results: Whole Mount Co-immunofluorescence Staining of Blood Vessels and Nerve Fibers in Adipose Tissues
- 11:17Conclusion
New blood vessel formation and sympathetic innervation play pivotal roles in adipose tissue remodeling. However, there remain technical issues in visualizing and quantitatively measuring adipose tissue. Here we present a protocol to successfully label and quantitatively compare the densities of blood vessels and nerve fibers in different adipose tissues.
