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JoVE Journal
Immunology and Infection

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Protocol for Recombinant RBD-based SARS Vaccines

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

Article DOI: 10.3791/2444
May 2nd, 2011


Summary May 2nd, 2011

This protocol describes a general procedure for studying recombinant receptor-binding domain (RBD)-based subunit vaccines against SARS. It includes methods for transfection and expression of RBD protein in 293T cells, immunization of mice with RBD and detection of neutralization activity of mouse sera using an established SARS pseudovirus neutralization assay.

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