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Using Caenorhabditis elegans for Studying Trans- and Multi-Generational Effects of Toxicants
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JoVE Journal Environment
Using Caenorhabditis elegans for Studying Trans- and Multi-Generational Effects of Toxicants

Using Caenorhabditis elegans for Studying Trans- and Multi-Generational Effects of Toxicants

DOI:
10.3791/59367-v

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08:58 min

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July 29, 2019

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, , , ,
1College of Environmental Sciences and Engineering,Tongji University, 2Shanghai Institute of Pollution Control and Ecological Security, 3Jiaxing Tongji Institute of Environment, 4College of Ecological Technique and Engineering,Shanghai Institute of Technology

Chapters

  • 00:04Title
  • 01:16Culture E. coli OP50 and C. elegans
  • 02:00Prepare Synchronized Eggs and L3 Larvae of C. elegans
  • 04:12Use C. elegans for Trans-generational Effect Study
  • 05:58Use C. elegans for Multi-generational Exposure (MGE) Effect Study
  • 07:04Results: Trans-generational (TG), Multi-generational Exposure (MGE) and Multi-generational Residual (MGR) Effect Studies
  • 08:08Conclusion

Summary

Automatic Translation

Automatic Translation

Trans- and multi-generational effects of persistent chemicals are essential in judging their long-term consequences in the environment and on the human health. We provide novel detailed methods for studying trans- and multi-generational effects using free-living nematode Caenorhabditis elegans.

Tags

Caenorhabditis ElegansTrans-generational EffectsMulti-generational EffectsToxicantsNematodeHermaphroditesPharmaceutical SynthesisEnvironmental ManagementAquatic ToxicityDaphnia MagnaDrosphila MelanogasterE. ColiLB MediumNGM AgarsGravid NematodesAge-synchronizationSodium Hypochlorite Solution

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