Journal
/
Medicine
/
Direct Intrabronchial Administration to Improve the Selective Agent Deposition Within the Mouse Lung
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Direct Intrabronchial Administration to Improve the Selective Agent Deposition Within the Mouse Lung

Direct Intrabronchial Administration to Improve the Selective Agent Deposition Within the Mouse Lung

DOI:
10.3791/59450-v

•

07:10 min

•

May 20, 2019

•

, , ,
1Department of Medicine,University of Colorado Denver, 2Department of Medicine,Denver Health Medical Center

Chapters

  • 00:04Title
  • 01:09Non-Operative Intratracheal (IT) Intubation
  • 02:16Intrabronchial (IB) Approach to Distal Right Lung Selective Lobar Cannulation
  • 03:00IB Approach to Distal Left Lung Selective Segmental Cannulation
  • 03:44Right Entire Lung Administration
  • 04:37Left Entire Lung Administration
  • 05:31Results: Representative Effects of Direct IB Selective Agent Administration to Whole Mouse Lung
  • 06:49Conclusion

Summary

Automatic Translation

Automatic Translation

Intratracheal (IT) administration of experimental agents in mice often results in asymmetric delivery to the distal lungs.  In this report, we describe a direct intrabronchial (IB) approach to cannulate each lung in living mice non-operatively.  This approach can be used to selectively administer agents to one lung or may be adapted to improve the symmetric agent delivery to both lungs.

Tags

Keywords: Intrabronchial AdministrationSelective Agent DepositionMouse LungIntratracheal InjectionUnilateral AdministrationCatheter PreparationAnesthetized MouseC57-Black/6 MouseEvans Blue DyeSelective Lobar CannulationDistal Right LungDistal Left Lung

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59450?article_type=v

High-frequency High-resolution Echocardiography: First Evidence on Non-invasive Repeated Measure of Myocardial Strain, Contractility, and Mitral Regurgitation in the Ischemia-reperfused Murine Heart
check_url/59450?article_type=v

Bronchial Thermoplasty: A Novel Therapeutic Approach to Severe Asthma
check_url/59450?article_type=v

Noninvasive Intratracheal Intubation to Study the Pathology and Physiology of Mouse Lung
check_url/59450?article_type=v

Diagnostic Ultrasound Imaging of Mouse Diaphragm Function
check_url/59450?article_type=v

The Bovine Lung in Biomedical Research: Visually Guided Bronchoscopy, Intrabronchial Inoculation and In Vivo Sampling Techniques
check_url/59450?article_type=v

Visualization of Streptococcus pneumoniae within Cardiac Microlesions and Subsequent Cardiac Remodeling
check_url/59450?article_type=v

Cardiac Catheterization in Mice to Measure the Pressure Volume Relationship: Investigating the Bowditch Effect
check_url/59450?article_type=v

Design, Fabrication, and Administration of the Hand Active Sensation Test (HASTe)
check_url/59450?article_type=v

2-Methacryloyloxyethyl Phosphorylcholine Polymer Treatment of Complete Dentures to Inhibit Denture Plaque Deposition
check_url/59450?article_type=v

Quantitative Mapping of Specific Ventilation in the Human Lung using Proton Magnetic Resonance Imaging and Oxygen as a Contrast Agent

Read Article