Name: Design of an Open-Source, Low-Cost Bioink and Food Melt Extrusion 3D Printer (Video) | JoVE Journal
Uploaded: 2020-03-02T12:00:00.000+00:00
Duration: 8 min 1 s
Description: Learn how to construct ADDME, a low-cost open-source melt extrusion 3D printer for biomedical and food printing applications. This method enables affordable access to complex 3D geometry production.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

This research did not receive any specific grants from funding agencies in the public, commercial, or not-for-profit sectors. Special thanks to Florian Schmittner, Sandro Gorka, Gurinder Singh, Vincent Tran, and Dominik Vu for their contribution on an earlier prototype of the design.

<ol><li>Brettel, M., Friederichsen, N., Keller, M., Rosenberg, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((How+Virtualization%2C+Decentralization+and+Network+Building+Change+the+Manufacturing+Landscape%3A+An+Industry+4.0+Perspective%5BTitle%5D)+AND+%22World+Academy+of+Science%2C+Engineering+and+Technology+International+Journal+of+Information+and+Communication+Engineering%22%5BJournal%5D)">How Virtualization, Decentralization and Network Building Change the Manufacturing Landscape: An Industry 4.0 Perspective.</a> World Academy of Science, Engineering and Technology International Journal of Information and Communication Engineering. 8 (1), (2014).</li>
<li>Gilchrist, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Introducing+Industry+4.0%5BTitle%5D)+AND+%22Industry+4.0%22%5BJournal%5D)">Introducing Industry 4.0.</a> Industry 4.0. , 195-215 (2016).</li>
<li>Petrick, I. J., Simpson, T. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((3D+Printing+Disrupts+Manufacturing%3A+How+Economies+of+One+Create+New+Rules+of+Competition%5BTitle%5D)+AND+%22Research-Technology+Management%22%5BJournal%5D)">3D Printing Disrupts Manufacturing: How Economies of One Create New Rules of Competition.</a> Research-Technology Management. 56 (6), 12-16 (2013).</li>
<li>Wong, K., Hernandez, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+Review+of+Additive+Manufacturing%5BTitle%5D)+AND+%22ISRN+Mechanical+Engineering%22%5BJournal%5D)">A Review of Additive Manufacturing.</a> ISRN Mechanical Engineering. 10, (2012).</li>
<li>Lanaro, M., Desselle, M. R., Woodruff, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((3D+Printing+Chocolate%3A+Properties+of+Formulations+for+Extrusion%2C+Sintering%2C+Binding+and+Ink+Jetting%5BTitle%5D)+AND+%22Fundamentals+of+3D+Food+printing+and+Applications%22%5BJournal%5D)">3D Printing Chocolate: Properties of Formulations for Extrusion, Sintering, Binding and Ink Jetting.</a> Fundamentals of 3D Food printing and Applications. , (2018).</li>
<li>Godoi, F. C., Prakash, S., Bhandari, B. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((3d+printing+technologies+applied+for+food+design%3A+Status+and+prospects%5BTitle%5D)+AND+%22Journal+of+Food+Engineering%22%5BJournal%5D)">3d printing technologies applied for food design: Status and prospects.</a> Journal of Food Engineering. 179, 44-54 (2016).</li>
<li>Stansbury, J. W., Idacavage, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((3D+printing+with+polymers%3A+Challenges+among+expanding+options+and+opportunities%5BTitle%5D)+AND+%22Dental+Materials%22%5BJournal%5D)">3D printing with polymers: Challenges among expanding options and opportunities.</a> Dental Materials. 32 (1), 54-64 (2016).</li>
<li>Zhu, W., Ma, X., Gou, M., Mei, D., Zhang, K., Chen, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((3D+printing+of+functional+biomaterials+for+tissue+engineering%5BTitle%5D)+AND+%22Current+Opinion+in+Biotechnology%22%5BJournal%5D)">3D printing of functional biomaterials for tissue engineering.</a> Current Opinion in Biotechnology. 40, 103-112 (2016).</li>
<li>Lanaro, M., Booth, L., Powell, S. K., Woodruff, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Electrofluidodynamic+technologies+for+biomaterials+and+medical+devices%3A+melt+electrospinning%5BTitle%5D)+AND+%22Electrofluidodynamic+Technologies+%28EFDTs%29+for+Biomaterials+and+Medical+Devices%22%5BJournal%5D)">Electrofluidodynamic technologies for biomaterials and medical devices: melt electrospinning.</a> Electrofluidodynamic Technologies (EFDTs) for Biomaterials and Medical Devices. , 37-69 (2018).</li>
<li>Malone, E., Lipson, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Fab%40Home%3A+the+personal+desktop+fabricator+kit+Article+information%5BTitle%5D)+AND+%22Rapid+Prototyping+Journal%22%5BJournal%5D)">Fab@Home: the personal desktop fabricator kit Article information.</a> Rapid Prototyping Journal. 13 (4), 245-255 (2007).</li>
<li>Vilbrandt, T., Malone, E., Lipson, H., Pasko, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Universal+Desktop+Fabrication%5BTitle%5D)+AND+%22Heterogeneous+Objects+Modelling+and+Applications%22%5BJournal%5D)">Universal Desktop Fabrication.</a> Heterogeneous Objects Modelling and Applications. , 259-284 (2008).</li>
<li>Jones, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((RepRap-the+replicating+rapid+prototyper%5BTitle%5D)+AND+%22Robotica%22%5BJournal%5D)">RepRap-the replicating rapid prototyper.</a> Robotica. 29, 177-191 (2011).</li>
<li>Lanaro, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((3D+printing+complex+chocolate+objects%3A+Platform+design%2C+optimization+and+evaluation%5BTitle%5D)+AND+%22Journal+of+Food+Engineering%22%5BJournal%5D)">3D printing complex chocolate objects: Platform design, optimization and evaluation.</a> Journal of Food Engineering. , (2017).</li>
<li>Wu, W., DeConinck, A., Lewis, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Omnidirectional+Printing+of+3D+Microvascular+Networks%5BTitle%5D)+AND+%22Advanced+Materials%22%5BJournal%5D)">Omnidirectional Printing of 3D Microvascular Networks.</a> Advanced Materials. 23 (24), H178-H183 (2011).</li>
<li>Paxton, N., Smolan, W., Böck, T., Melchels, F., Groll, J., Jungst, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Proposal+to+assess+printability+of+bioinks+for+extrusion-based+bioprinting+and+evaluation+of+rheological+properties+governing+bioprintability%5BTitle%5D)+AND+%22Biofabrication%22%5BJournal%5D)">Proposal to assess printability of bioinks for extrusion-based bioprinting and evaluation of rheological properties governing bioprintability.</a> Biofabrication. 9 (4), 044107(2017).</li>
</ol>

The authors have nothing to disclose.

This protocol provides detailed instructions for constructing a low-cost melt extrusion-based 3D printer. Construction of the 3D printer can be broken down into subsections including frame, y-axis/bed, x-axis, extruder, electronics, and software. These subsections are presented with detailed diagrams, drawings, files and parts lists. The total price of an ADDME 3D printer comes to $343 AUD ($245 USD as of 01/17/2019), making this the cheapest, reservoir-based melt extrusion 3D printer currently known. It was aimed to mak...

Additive manufacturing is a powerful manufacturing technology that has the potential to provide significant value to the industrial landscape1,2. The attractive features of additive manufacturing involve no tooling costs, high levels of customization, complex geometries, and reduced barriers to entry costs. No retooling costs allow for the rapid manufacturing of prototypes, which is desirable when trying to decrease &#34;time to market&#34;, which is a critical aim of industries in developed nations trying to remain competitive against low-wage competitors1. High levels of customizability allow for a wide variety of products to be fabricated with complex geometries. When these factors are combined with the low costs for set-up, materials, and operator specialization, there is a clear value of additive manufacturing technologies3.
Additive manufacturing, also called 3D printing, involves layer-by-layer fabrication of an object in a computer numerical controlled (CNC) system3. Unlike traditional CNC processes such as milling, in which material is removed from a sheet or block of material, a 3D printing system adds material into the desired structure layer-by-layer.
3D printing can be facilitated through a range of methods including laser, flash, extrusion, or jetting technologies4. The specific technology employed determines the form of the raw material (i.e., powder or melt), as well as the rheological and thermal properties required for processing5. The extrusion-based 3D printing market is dominated by filament-based systems, which is due to filaments being easy to handle, process, and continuously supply large volumes of material to the extrusion head. However, this process is limited by the type of material able to be formed into filaments (mainly thermoplastics). Most materials do not exist in filament form, and the lack of modern low-cost platforms in the market represents a notable gap.
This protocol shows the construction of a reservoir-based extrusion system that allows materials to be stored in a syringe and extruded through a needle. This system is ideally suited to manufacture a wide range of materials including foods6, polymers7, and biomaterials8,9. Furthermore, reservoir-based extrusion techniques are typically less hazardous, lower in cost, and easier to operate than other 3D printing methods.
There is a growing number of university-led teams designing and releasing open-source 3D printing systems to the public. Beginning with the Fab@Home extrusion-based printer in 200710,11, researchers aimed to create a simple and cheap platform to drive rapid expansion in 3D printing technology and applications. Later in 2011, the RepRap project aimed to create a filament-based 3D printing platform designed with parts made by 3D printing, with the goal to create a self-replicating machine12. The cost of 3D printers has been dropping over the years, from $2300 USD for a Fab@Home (2006), $573 USD for a RepRap v1 (2005), and $400 USD for v2 (2011).
In previous work, we demonstrated how an off-the-self 3D printing system could be combined with a custom reservoir-based extrusion system to create complex 3D objects from chocolate13. Further design investigation has shown that considerable cost savings can be achieved compared to this prototype design.
The aim of this protocol is to provide instructions for the construction of a low-cost reservoir-based melt extrusion 3D printer. Presented here are detailed diagrams, drawings, files, and component lists to allow successfully construction and operation of a 3D printer. All components are hosted on the open-source (creative commons noncommercial) platform <a href="https://www.thingiverse.com/Addme/collections" target="_blank">https://www.thingiverse.com/Addme/collections</a>, which allows users to change or add additional features as desired. Viscous cream, chocolate, and Pluronic F-127 (a model for bioinks) are used to evaluate the performance of ADDME and demonstrate application of the ADDME 3D printer to the biomedical and food printing industries.
A laser cutter capable of cutting acrylic and a desktop 3D printer capable of printing PLA or ABS filaments are required for this protocol. A machined heating jacket and heater cartridge or silicone heater can be used to heat the material, depending on which equipment the operator has access to. All CAD files can be found at <a href="https://www.thingiverse.com/Addme/designs" target="_blank">https://www.thingiverse.com/Addme/designs</a>. For firmware and software to control the 3D printer, <a href="http://marlinfw.org/meta/download/" target="_blank">http://marlinfw.org/meta/download/</a> and <a href="https://www.repetier.com/" target="_blank">https://www.repetier.com/</a> are provided resources, respectively. For detailed instructions about the control board, see <a href="https://reprap.org/wiki/RAMPS_1.4" target="_blank">https://reprap.org/wiki/RAMPS_1.4</a>.

<table><tbody><tr><td>15 W 12V DC 50x100mm Flexible Silicon Heater</td><td>Banggood</td><td>1280175</td><td>Optional; AU$4.46</td></tr><tr><td>3D Printer</td><td>Lulzbot</td><td></td><td>&lt;a href=&quot;https://download.lulzbot.com/TAZ/6.02/documentation/manual/9780989378482_interior_r6.02.pdf&quot; target=&quot;_blank&quot;&gt;https://download.lulzbot.com/&lt;/a&gt;</td></tr><tr><td>3D Printer</td><td>Ultimaker</td><td>Ultimaker 2+</td><td></td></tr><tr><td>AC 100-240V to DC 12V 5A 60W Power Supply</td><td>Banggood</td><td>994870</td><td>AU$12.7</td></tr><tr><td>Acrylic Sheet White Continuous Cast 1200x600mm</td><td>Mulford Plastics</td><td></td><td>AU$36.95</td></tr><tr><td>Allen Keys</td><td>Metric</td><td></td><td></td></tr><tr><td>Arduino MEGA2560 R3 with RAMPS 1.4 Controller</td><td>Geekcreit</td><td>984594</td><td>AU$28.91</td></tr><tr><td>Carbon Steel Linear Shaft 8mm x 350mm</td><td>Banggood</td><td>1119330</td><td>AU$13.44</td></tr><tr><td>Carbon Steel linear Shaft 8mm x 500mm</td><td>Banggood</td><td>1276011</td><td>AU$19.42</td></tr><tr><td>Chocolate</td><td>Cadbury</td><td></td><td></td></tr><tr><td>Computer with internet access</td><td>Dell</td><td></td><td></td></tr><tr><td>Coupler 5-8mm</td><td>Banggood</td><td>1070710</td><td>AU$6.93</td></tr><tr><td>Hand Cream</td><td>Nivea</td><td>80102</td><td></td></tr><tr><td>Heating Cartridge</td><td>Creality 3D</td><td>1192704</td><td>AU$4.75</td></tr><tr><td>K Type Temperature Sensor Thermocouple</td><td>Banggood</td><td>1212169</td><td>AU$2.37</td></tr><tr><td>Laser Cutter</td><td>trotec</td><td>Speedy 300</td><td>&lt;a href=&quot;https://www.troteclaser.com/fileadmin/content/images/Contact_Support/Manuals/8047-speedy-400-redesign-manual-EN.pdf&quot; target=&quot;_blank&quot;&gt;https://www.troteclaser.com/&lt;/a&gt;</td></tr><tr><td>M10 1mm Pitch Thread Metal Hex Nut + Washer</td><td>UXCELL</td><td></td><td>AU$8.84</td></tr><tr><td>M10 1mm Pitch Zinc Plated Pipe 400mm Length</td><td>UXCELL</td><td></td><td>AU$11.62</td></tr><tr><td>M2 - 0.4mm Internal Thread Brass Inserts</td><td>Ebay</td><td></td><td>AU$5.65</td></tr><tr><td>M2 Nuts</td><td>Suleve</td><td>1239291</td><td>AU$9.17</td></tr><tr><td>M2 x 10 mm Button Hex Screws</td><td>Suleve</td><td>1239291</td><td>AU$9.17</td></tr><tr><td>M2 x 5mm Button Hex Screws</td><td>Suleve</td><td>1239291</td><td>AU$9.17</td></tr><tr><td>M3 - 0.5mm Internal Thread Brass Inserts</td><td>Suleve</td><td>1262071</td><td>AU$7.5</td></tr><tr><td>M3 Nuts</td><td>Suleve</td><td>1109208</td><td>AU$7.85</td></tr><tr><td>M3 Washer</td><td>Banggood</td><td>1064061</td><td>AU$3.05</td></tr><tr><td>M3 x 10mm Button Hex Screws</td><td>Suleve</td><td>1109208</td><td>AU$7.85</td></tr><tr><td>M3 x 20mm Button Hex Screws</td><td>Suleve</td><td>1109208</td><td>AU$7.85</td></tr><tr><td>M3 x 6mm Button Hex Screws</td><td>Suleve</td><td>1109208</td><td>AU$7.85</td></tr><tr><td>M3 x 8mm Button Hex Screws</td><td>Suleve</td><td>1109208</td><td>AU$7.85</td></tr><tr><td>M4 x 8mm Button Hex Screws</td><td>Suleve</td><td>1273210</td><td>AU$4.32</td></tr><tr><td>Needle Luer Lock 18 - 27 Gauge</td><td>Terumo</td><td>TGA ARTG ID: 130227</td><td>AU$3.57</td></tr><tr><td>NEMA 17 Stepper Motor</td><td>Casun</td><td>42SHD0001-24B</td><td>AU$54</td></tr><tr><td>NEMA Stepper Motor Mounting Bracket</td><td>Banggood</td><td>ptNema17br90</td><td>AU$4.79</td></tr><tr><td>Pillow Block Flange Bearing 8mm</td><td>Banggood</td><td>KFL08</td><td>AU$5.04</td></tr><tr><td>PLA Filament</td><td>Creality 3D</td><td>1290153</td><td>AU$24.95</td></tr><tr><td>Pluronic F127</td><td>Sigma Aldrich</td><td>P2443-250G</td><td></td></tr><tr><td>SC8UU 8mm Linear Motion Ball Bearing</td><td>Toolcool</td><td>935967</td><td>AU$21.6</td></tr><tr><td>SG-5GL Micro Limit Switch</td><td>Omron</td><td>1225333</td><td>AU$4.5</td></tr><tr><td>Soldering Station</td><td></td><td></td><td>Solder, Wires, Heat shrink e.c.t.</td></tr><tr><td>Spring</td><td>Banggood</td><td>995375</td><td>AU$2.53</td></tr><tr><td>Syringe 3ml Luer Lock Polypropylene</td><td>Brauhn</td><td>9202618N</td><td>AU$3.14</td></tr><tr><td>Timing Pulley GT2 20 Teeth and Belt Set</td><td>Banggood</td><td>10811303</td><td>AU$11.48</td></tr><tr><td>Trapezoidal Lead Screw and Nut 8mm x 400mm</td><td>Banggood</td><td>1095315</td><td>AU$29.02</td></tr><tr><td>Variable Spanner</td><td></td><td></td><td></td></tr></tbody></table>

design of an open-source, low-cost bioink and food melt extrusion 3d printer

Design of an Open-Source, Low-Cost Bioink and Food Melt Extrusion 3D Printer

Three-dimensional (3D) printing is an increasingly popular manufacturing technique that allows highly complex objects to be fabricated with no retooling costs. This increasing popularity is partly driven by falling barriers to entry such as system set-up costs and ease of operation. The following protocol presents the design and construction of an Additive Manufacturing Melt Extrusion (ADDME) 3D printer for the fabrication of custom parts and components. ADDME has been designed with a combination of 3D-printed, laser-cut, and online-sourced components. The protocol is arranged into easy-to-follow sections, with detailed diagrams and parts lists under the headings of framing, y-axis and bed, x-axis, extrusion, electronics, and software. The performance of ADDME is evaluated through extrusion testing and 3D printing of complex objects using viscous cream, chocolate, and Pluronic F-127 (a model for bioinks). The results indicate that ADDME is a capable platform for the fabrication of materials and constructs for use in a wide range of industries. The combination of detailed diagrams and video content facilitates access to low-cost, easy-to-operate equipment for individuals interested in 3D printing of complex objects from a wide range of materials.

The performance of ADDME during 3D printing was evaluated using a viscous cream (150 mL, Nivea hand cream), chocolate (Cadbury, plain milk), and Pluronic F-127 (Sigma Aldrich). The viscous cream and chocolate were used as is, and the Pluronic was dissolved into a 20% wt solution with ultrapure water and stored refrigerated at 5 °C until needed14,15.
Line testing involved...

The aim of this work is to design and construct a reservoir-based melt extrusion three-dimensional printer made from open-source and low-cost components for applications in the biomedical and food printing industries.

Three-dimensional (3D) printing is an increasingly popular manufacturing technique that allows highly complex objects to be fabricated with no retooling ...

design-an-open-source-low-cost-bioink-food-melt-extrusion-3d

Research

JoVE Journal

Bioengineering

A bioprinter was used to create patterned hydrogels based on a sacrificial mold.  The poloxamer mold was backfilled with a second hydrogel and then eluted, leaving voids which were filled with a third hydrogel. This method uses fast elution and good printability of poloxamer to generate complex architectures from biopolymers.

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

We have devised a method for low-cost and rapid prototyping of liquid elastomer rubber injection molded devices by using fused deposition modeling 3D printers for mold design and a modified desiccator as a liquid injection system.

Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding

Here we present a protocol to construct a pressure-controlled syringe pump to be used in microfluidic applications. This syringe pump is made from an additively manufactured body, off-the-shelf hardware, and open-source electronics. The resulting system is low-cost, straightforward to build, and delivers well-regulated fluid flow to enable rapid microfluidic research.

Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications

This article describes a method of transforming a low-cost commercial 3D printer into a bacterial 3D printer that can facilitate printing of patterned biofilms. All necessary aspects of preparing the bioprinter and bio-ink are described, as well as verification methods to assess the formation of biofilms.

Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter

Presented here is a method for the 3D bioprinting of gelatin methacryloyl.

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks

In this manuscript, we demonstrate the preparation of a biohybrid hydrogel bioink containing graphene for use in peripheral tissue engineering. Using this 3D biohybrid material, the neural differentiation protocol of stem cells is performed. This can be an important step in bringing similar biomaterials to the clinic.

Preparation and Characterization of Graphene-Based 3D Biohybrid Hydrogel Bioink for Peripheral Neuroengineering

Engineered flaps require an incorporated functional vascular network. In this protocol, we present a method of fabricating a 3D printed tissue flap containing a hierarchical vascular network and its direct microsurgical anastomoses to rat femoral artery.

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies

Shear processing during hydrogel formation results in the production of microgel suspensions that shear-thin but rapidly restructure following the removal of shear forces. Such materials have been used as a supporting matrix for bioprinting complex, cell-laden structures. Here, methods used to manufacture the supporting bed and compatible bioinks are described.

Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting

This study introduces a novel κ-carrageenan sub-microgel suspension bath, displaying remarkable reversible jamming-unjamming transition properties. These attributes contribute to the construction of biomimetic tissues and organs in embedded 3D bioprinting. The successful printing of heart/esophageal-like tissues with high resolution and cell growth demonstrates high-quality bioprinting and tissue engineering applications.

Embedded Bioprinting of Tissue-like Structures Using &#954;-Carrageenan Sub-Microgel Medium

Soluble basement membrane extracts are the most widely used biological matrices in cancer research, but their complex rheological behaviour makes their bioprinting difficult with commercially available bioprinting systems. This work presents a customized bioprinting strategy to produce pure matrix constructs with good shape fidelity in both single and multiple layers.

Design and Validation of a Volumetric-extrusion Bioprinter for Bioprinting of Soluble Basement Membrane Extract for Translational Research

Learn how to construct ADDME, a low-cost open-source melt extrusion 3D printer for biomedical and food printing applications. This method enables affordable access to complex 3D geometry production.

Design of an Open-Source, Low-Cost Bioink and Food Melt Extrusion 3D Printer

Explore More Videos