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脑毛细管中培养细胞细胞钙测量和钙成像研究
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies
DOI:

09:33 min

May 27, 2020

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Chapters

  • 00:04Introduction
  • 00:41Isolation of Primary Pericytes from Bovine Brain Capillaries
  • 03:01Loading of Pericytes with Calcium Indicator Dye in a Plate Reader Setup
  • 05:18Live Imaging of Intracellular Ca2+ Levels
  • 07:11Results: Pericyte Isolation and Calcium Measurements
  • 09:02Conclusion

Summary

Automatic Translation

脑毛细管性信号是调节血脑屏障特性和血流的基本参与者。该协议描述了如何分离、培养脑毛细管腹膜,在细胞类型方面进行特征分析,并应用于用荧光探针研究细胞内钙信号。

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