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Neuroscience
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脑毛细管中培养细胞细胞钙测量和钙成像研究
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies
Please note that all translations are automatically generated.
Click here for the English version.
脑毛细管中培养细胞细胞钙测量和钙成像研究
DOI:
10.3791/61253-v
•
09:33 min
•
May 27, 2020
•
Sofie Hørlyck
,
Hans Christian Cederberg Helms
,
Birger Brodin
1
Department of Pharmacy, The Faculty of Health and Medical Sciences
,
University of Copenhagen
Chapters
00:04
Introduction
00:41
Isolation of Primary Pericytes from Bovine Brain Capillaries
03:01
Loading of Pericytes with Calcium Indicator Dye in a Plate Reader Setup
05:18
Live Imaging of Intracellular Ca
2+
Levels
07:11
Results: Pericyte Isolation and Calcium Measurements
09:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
脑毛细管性信号是调节血脑屏障特性和血流的基本参与者。该协议描述了如何分离、培养脑毛细管腹膜,在细胞类型方面进行特征分析,并应用于用荧光探针研究细胞内钙信号。
Tags
Brain Capillary Pericytes
Primary Cell Culture
Calcium Imaging
Intracellular Signaling
Cell Isolation
Cell Detachment
DMEM
Trypsin-EDTA
Centrifugation
Cell Confluency
Microscopy
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