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Biology

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Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy
 

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy

Article doi: 10.3791/56832
January 18th, 2018

Summary January 18th, 2018

Viral transcriptional factories are discrete structures that are enriched with cellular RNA polymerase II to increase viral gene transcription during reactivation. Here, a method to locate sites of actively transcribing viral chromatin in 3D nuclear space by a combination of immunofluorescence staining and in situ RNA hybridization is described.

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