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Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy

DOI:
10.3791/2061-v

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13:08 min

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October 07, 2010

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, , , , ,
1Division of Infectious Diseases, Department of Medicine, Immunology Institute,Mount Sinai School of Medicine , 2NSF Center for Biophotonics,University of California, Davis, 3Structural and Computational Biology Unit,European Molecular Biology Laboratory

Chapters

  • 00:00Title
  • 00:55Introduction
  • 01:26Preparing the Donor (Jurkat) and Target (Primary) CD4+ T cells
  • 03:47Cleaning Up the Donor Cells and Labeling the Target Cells
  • 06:36Monitor Cell-to-cell Transfer of HIV Gag-iGFP Using Live Cell Transfer
  • 12:02Representative Images of Cell-to-cell Transfer of HIV
  • 12:29Conclusion

Summary

Automatic Translation

Automatic Translation

This visualized experiment is a guide for utilizing a fluorescent molecular clone of HIV for live confocal imaging experiments.

Tags

VisualizingCell-to-cell TransferHIVFluorescent ClonesLive Confocal MicroscopyGreen Fluorescent ProteinComplex Cellular ProcessesFluorescence Video MicroscopyHuman Immunodeficiency Virus Core ProteinGag ProteinInfectious Molecular CloneHIV Gag-iGFPVideo Confocal MicroscopyTransfectHuman T Cell LineFluorescently Labeled Uninfected CD4+ T CellsTarget CellsViral ProductionTransportIntercellular StructuresVirological SynapsesGas Permeable Imaging Chambers

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