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Chapters
- 00:00Introduciton
- 01:29Panels Preparation
- 02:49Drug 1, Cefotaxime, Serial Dilution
- 04:06Drug 2, Amikacin, Serial Dilution
- 05:33Transfer of Drug 2 to the Four Panels
- 05:56Drug 3, Levofloxacin, Addition
- 06:55Drug 4, Trimethoprim- Sulfamethoxazole, Addition
- 07:48Preparation and Addition of Bacterial Inoculum E.coil ESBL
- 08:55Results
- 10:53Conclusion
This protocol describes how to study all possible combinations that can be obtained between four drugs in one single experiment. This method is based on the standard 96-well plate micro dilution assay and the calculation of fractional inhibitory concentrations (FICs) to evaluate the results.
