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Diagonal Method to Measure Synergy Among Any Number of Drugs
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Diagonal Method to Measure Synergy Among Any Number of Drugs

Diagonal Method to Measure Synergy Among Any Number of Drugs

DOI:
10.3791/57713-v

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12:08 min

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June 21, 2018

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, , ,
1Faculty of Engineering and Natural Sciences,Sabanci University, 2Nanotechnology Research and Application Center,Sabanci University, 3Laboratory of Systems Pharmacology,Harvard Medical School

Chapters

  • 00:04Title
  • 00:28Serial Dilution Dose-Response Experiment
  • 04:29Linear Dilution Dose-Response Experiment
  • 07:21Diagonal Drug Interaction Experiment and Interaction Scores
  • 09:51Results: Analysis of the Diagonal Drug Interaction Experiment
  • 11:11Conclusion

Summary

Automatic Translation

Automatic Translation

In this protocol, we describe how to make Loewe additivity-based drug interaction measurements for pairwise and three-way drug combinations.

Tags

Keywords: Diagonal MethodSynergyMultiple DrugsSystems BiologyAntibiotic AliquotsBacterial CultureSerial DilutionsLB MediaDMSOLEVNALPNGOD600Dose-response ExperimentIC50

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