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JoVE Journal
Biochemistry

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Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block
 

Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block

Article DOI: 10.3791/62321-v 06:10 min April 8th, 2021
April 8th, 2021

Chapters

Summary

Sample preparation for cryo-electron microscopy (cryo-EM) is a significant bottleneck in the structure determination workflow of this method. Here, we provide detailed methods for using an easy-to-use, three-dimensionally printed block for the preparation of support films to stabilize samples for transmission EM studies.

Transcript

Tags

Cryo-EM Samples Support Films Flotation Block Sample Preparation Transmission Electron Microscopy Air-water Interface Denaturation Hydrophilic Treatment TEM Grids Plasma Cleaning Buffer Exchange Well Urinal Acetate Solution Negative Staining Mica Fragments Carbon Film
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