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Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy
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Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy

Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy

English

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2,294 Views

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08:05 min

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May 27, 2021

DOI:

10.3791/62658-v

DOI:
10.3791/62658-v

•

08:05 min
May 27, 2021

•

26 Views
, , ,
1Vascular Biology Center,Medical College of Georgia at Augusta University, 2Department of Cellular Biology and Anatomy,Medical College of Georgia at Augusta University, 3Department of Pharmacology and Toxicology,Medical College of Georgia at Augusta University

Summary

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Macropinocytosis is a highly conserved endocytic process initiated by the formation of F-actin-rich sheet-like membrane projections, also known as membrane ruffles. Increased rate of macropinocytotic solute internalization has been implicated in various pathological conditions. This protocol presents a method to quantify membrane ruffle formation in vitro using scanning electron microscopy.

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Cite this Article

Ahn, W., Singla, B., Marshall, B., Csányi, G. Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy. J. Vis. Exp. (171), e62658, doi:10.3791/62658 (2021).

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