Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing

This method of retinal endothelial cell isolation will allow for advanced sequencing techniques to reveal novel mechanisms of vascular development. The described protocol is optimized for high degrees of viability and purity, which are essential for next-generation sequencing applications. Demonstrating the procedure will be Shelby Cain, a graduate student from the Hershey Laboratory.

Start removing eyes from the euthanized neonatal mouse by cutting away the skin and membrane over the eye with a perpendicular incision to the eyelid using dissection scissors. Then use the forceps to press down above and below the eye so that the eye moves out of the socket. Carefully pinch underneath the eye with the forceps and cut the optic nerve that keeps the eye attached.