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JoVE Journal

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Isolation of Murine Valve Endothelial Cells

Isolation of Murine Valve Endothelial Cells

Article DOI: 10.3791/51860
August 21st, 2014


Summary August 21st, 2014

The ability to isolate heart valve endothelial cells (VECs) is critical for understanding mechanisms of valve development, maintenance, and disease. Here we describe the isolation of VECs from embryonic and adult Tie2-GFP mice using FACS that will allow for studies determining the contribution of VECs in developmental and disease processes.

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