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Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure (Video) | JoVE
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Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure

Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure (Video) | JoVE

Direct Synthesis of Gold Nanoparticles for Protein Localization Analysis

DOI:
10.3791/65246-v

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01:42 min

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April 28, 2023

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1National Institute of Biological Sciences, Beijing, 2Tsinghua Institute of Multidisciplinary Biomedical Research,Tsinghua University

Tags

Keywords: EM-visible Gold NanoparticlesProtein Localization AnalysisUltrastructure PreservationGenetically-encoded TagsCysteine-rich TagsMetallothionein (MT)Antifreeze Protein (AFP)Autonucleation Suppression Mechanism (ANSM)High-pressure FreezingFreeze-substitution FixationNon-aldehyde FixativesElectron Microscopy Labeling

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