Direct Synthesis of Gold Nanoparticles for Protein Localization Analysis

April 28, 2023

Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure (Video) | JoVE

01:42 min

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ï»¿Preparing Sapphire Discs for Cell Culture (Video) | JoVE

01:23 min

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High-Pressure Freezing (HPF), Freeze-Substitution Fixation (FSF) and Rehydration of Sapphire Disc-Carrier Assembly (Video) | JoVE

03:20 min

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Gold Nanoparticle Synthesis for Mammalian Cells Using Sapphire Discs (Video) | JoVE

03:23 min

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Zhaodi Jiang²

¹National Institute of Biological Sciences, Beijing, ²Tsinghua Institute of Multidisciplinary Biomedical Research,Tsinghua University

Summary

The present protocol describes a clonable electron microscopy labeling technology for detecting metallothionein-tagged proteins in cells using a novel autonucleation suppression mechanism-based gold nanoparticle synthesis technique.