Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure (Video) | JoVE

Zhaodi Jiang

doi:10.3791/65246

Direct Synthesis of Gold Nanoparticles for Protein Localization Analysis

April 28th, 2023

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Zhaodi Jiang^1,2

¹National Institute of Biological Sciences, Beijing, ²Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University

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The present protocol describes a clonable electron microscopy labeling technology for detecting metallothionein-tagged proteins in cells using a novel autonucleation suppression mechanism-based gold nanoparticle synthesis technique.

Article

Direct Synthesis of Gold Nanoparticles for Protein Localization Analysis

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