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Direct Synthesis of Gold Nanoparticles for Protein Localization Analysis

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在细胞中直接合成EM-可见金纳米颗粒，用于蛋白质定位分析，具有保存完好的超微结构

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Biology

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JoVE Journal Biology

Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure

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在细胞中直接合成EM-可见金纳米颗粒，用于蛋白质定位分析，具有保存完好的超微结构

Direct Synthesis of Gold Nanoparticles for Protein Localization Analysis

DOI:

10.3791/65246-v

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01:42 min

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April 28, 2023

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Zhaodi Jiang²

¹National Institute of Biological Sciences, Beijing, ²Tsinghua Institute of Multidisciplinary Biomedical Research,Tsinghua University

Tags

Keywords: EM-visible Gold Nanoparticles Protein Localization Analysis Ultrastructure Preservation Genetically-encoded Tags Cysteine-rich Tags Metallothionein (MT)Antifreeze Protein (AFP)Autonucleation Suppression Mechanism (ANSM)High-pressure Freezing Freeze-substitution Fixation Non-aldehyde Fixatives Electron Microscopy Labeling

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