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Journal
/
Biology
/
Harvesting Mouse Skeletal Muscle to Culture Stem Cells and Their Niche
/
Author Spotlight: Ex Vivo Protocol for Culturing Quiescent Muscle Stem Cells with Niche Components
JoVE Journal
Biology
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JoVE Journal
Biology
Unfractionated Bulk Culture of Mouse Skeletal Muscle to Recapitulate Niche and Stem Cell Quiescence
Author Spotlight: Ex Vivo Protocol for Culturing Quiescent Muscle Stem Cells with Niche Components
Harvesting Mouse Skeletal Muscle to Culture Stem Cells and Their Niche
DOI:
10.3791/65433-v
•
01:45 min
•
June 02, 2023
•
Louai Zaidan*
1
,
Perla Geara*
1
,
Matthew J. Borok*
1
,
Léo Machado
,
Despoina Mademtzoglou
,
Philippos Mourikis
,
Frederic Relaix
2,3,4
1
Univ Paris Est Creteil, INSERM, IMRB, F-94010 Creteil, France
,
2
Ecole nationale vétérinaire d’Alfort, IMRB, F-94700 Maisons-Alfort, France
,
3
EFS, IMRB, F-94010 Creteil, France
,
4
AP-HP, Hopital Mondor, Service d’histologie, F-94010 Creteil, France
Tags
Skeletal Muscle
Satellite Cells
Niche
Quiescence
Single-cell RNA Sequencing
FACS
In Vitro Culture
Myofibers
Stem Cells
Endothelial Cells
Fibro-adipogenic Progenitors
Regeneration
Homeostasis
Disease
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