Understanding the Changes in Mitochondrial Morphology through Dynamic and Three-dimensional Fluorescence Micrographs

The aim of our research is to observe changes in mitochondrial networks and investigate how these mitochondrial networks change in response to cellular conditions. We use open source tools like Fiji and Python, combining existing libraries with custom macros and scripts to automate the mitochondrial morphology analysis from complex fluorescence microscopy data. Achieving reliable quantitative data and metrics that describe the dynamics of mitochondrial fission and fusion with their localization in 3D remains a challenge.

Standardization for such data generation is not commonly available. Therefore, limited knowledge exists on cell specific fission and fusion frequency and intracellular localization. We added life detection of mitochondrial fission and fusion to the available research metrics.