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3D Mitochondrial Ultrastructure of Drosophila Indirect Flight Muscle Revealed by Serial-section Electron Tomography
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3D Mitochondrial Ultrastructure of Drosophila Indirect Flight Muscle Revealed by Serial-section Electron Tomography

3D Mitochondrial Ultrastructure of Drosophila Indirect Flight Muscle Revealed by Serial-section Electron Tomography

DOI:
10.3791/56567-v

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06:45 min

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December 19, 2017

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, ,
1Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine,National Taiwan University, 2Institute of Cellular and Organismic Biology,Academia Sinica

Chapters

  • 00:05Title
  • 00:37Sectioning Drosophila Tissues with Vibrating Blade Microtome
  • 01:19High-pressure Freezing (HPF) and Freeze Substitution (FS) for Electron Microscope (EM)
  • 02:30Serial-sectioning for Electron Tomography
  • 03:42Double-title Electron Tomography
  • 05:09Results: Integrated Intra-mitochondria Cristae and Matrix Patterns in 3D
  • 06:20Conclusion

Summary

Automatic Translation

Automatic Translation

In this protocol, we demonstrate the application of serial-section electron tomography to elucidate mitochondrial structure in Drosophila indirect flight muscle.

Tags

Keywords: 3DMitochondrial UltrastructureDrosophila Indirect Flight MuscleSerial-section Electron TomographyCellular UltrastructureHigh-pressure FreezingFree SubstitutionResin EmbeddingUltramicrotomyTransmission Electron MicroscopeDual-axis Tomography

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