Journal
/
Biology
/
Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue

Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue

DOI:
10.3791/63606-v

•

08:12 min

•

May 05, 2022

•

, , , , , , , , , , ,
1Department of Biochemistry,Albert Einstein College of Medicine

Chapters

  • 00:04Introduction
  • 00:53Preparation of the 3D Culture System
  • 02:20Growth of Spheroids in the Bioreactor
  • 03:15Histone Extraction
  • 04:27The First Round of Derivatization
  • 05:03Desalting, Sample Cleanup, and Sample Preparation for LC-MS/MS
  • 06:08Results: Identification and Quantification of Histone Post-Translational Modifications
  • 07:37Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol outlines how a three-dimensional cell culture system can be used to model, treat, and analyze chromatin modifications in a near-physiological state.

Tags

Keywords: 3D Cell CultureHistone Post-translational ModificationsLiquid Chromatography Mass SpectrometrySpheroid FormationBioreactor

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63606?article_type=v

Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture
check_url/63606?article_type=v

Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA
check_url/63606?article_type=v

Detection of Histone Modifications in Plant Leaves
check_url/63606?article_type=v

Isolation and Primary Culture of Rat Hepatic Cells
check_url/63606?article_type=v

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications
check_url/63606?article_type=v

Identification of Post-translational Modifications of Plant Protein Complexes
check_url/63606?article_type=v

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
check_url/63606?article_type=v

High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications
check_url/63606?article_type=v

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
check_url/63606?article_type=v

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells

Read Article