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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

DOI:
10.3791/63802-v

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06:13 min

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December 09, 2022

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, ,
1Department of Bioengineering,Northeastern University, 2Barnett Institute,Northeastern University, 3Department of Biology,Northeastern University

Chapters

  • 00:05Introduction
  • 00:47Single-Cell Proteomics Sample Preparation
  • 04:43Results: Single-Cell Proteomics Analysis
  • 05:27Conclusion

Summary

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In this protocol, we describe how to prepare mammalian cells for single-cell proteomics analysis via mass spectrometry using commercially available reagents and equipment, with options for both manual and automatic pipetting.

Tags

Keywords: Single-cell ProteomicsSCoPE2Mass SpectrometryFreeze-heat LysisIsobaric CarrierTrypsin DigestionTandem Mass TaggingLC-MS Analysis

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