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Hydroponics: Et alsidigt system til Uddannelse Næringsstof Fordeling og Plant Svar på Nutrient tilgængelighed og eksponering for giftige elementer

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Nguyen, N. T., McInturf, S. A., Mendoza-Cózatl, D. G. Hydroponics: A Versatile System to Study Nutrient Allocation and Plant Responses to Nutrient Availability and Exposure to Toxic Elements. J. Vis. Exp. (113), e54317, doi:10.3791/54317 (2016).

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Abstract

Hydroponiske systemer er blevet anvendt som en af ​​de standardmetoder til plantebiologi forskning og anvendes også i kommerciel produktion i flere afgrøder, herunder salat og tomat. Inden anlægget forskningsverdenen, har mange hydroponiske systemer er designet til at studere plante reaktioner på biotiske og abiotiske påvirkninger. Her præsenterer vi en hydroponiske protokol, der let kan implementeres i laboratorier interesseret i at forfølge undersøgelser af anlæg mineral ernæring.

Denne protokol beskriver den hydroponiske system, der oprettes i detaljer og udarbejdelse af plantemateriale for vellykkede eksperimenter. De fleste af de i denne protokol materialer kan findes uden videnskabelige forsyningsselskaber, hvilket gør den sat op til hydroponiske eksperimenter billigere og bekvemt.

Anvendelsen af ​​et hydroponiske vækst system er mest fordelagtig i situationer, hvor de næringsmedier skal velkontrolleret og når intakt roOTS skal høstes til efterfølgende anvendelser. Vi viser også, hvordan næringsstof koncentrationer kan ændres til at fremkalde plante svar på både essentielle næringsstoffer og giftige ikke-væsentlige bestemmelser.

Materials

Name Company Catalog Number Comments
For seed sterilization
Bleach The Clorox Company NA The regular bleach
www.cloroxprofessional.com
Hydrochloric acid Fisher Scientific A144-500
Desiccator body Nalgene D2797 SIGMA Marketed by Sigma-Aldrich
Desiccator plate Nalgene 5312-0230 Marketed by Thermo Scientific
For one quarter MS medium preparation
MES Acros Organics 172591000 4-Morpholineethanesulfonic acid hydrate
Murashige and Skoog (MS) Sigma-Aldrich M0404-10L
KOH Fisher Scientific P250-500
Phytoagar Duchefa Biochemie P1003.1000
Square plate Fisher Scientific 0875711A Disposable Petri Dish With Grid
For seed plating 
Filter paper Whatman 1004090
Toothpick Jarden Home Brands NA
Aluminum foil Reynolds Wrap NA Standard aluminum foil
Micropore tape 3M Health Care 19-898-074 Surgical tape; Marketed by Fisher Scientific
For hydroponic solution preparation
KNO3 Fisher Scientific  BP368-500
KH2PO4 Fisher Scientific P386-500
MgSO4 Fisher Scientific M63-500
Ca(NO3)2 Acros Organics A0314209
H3BO3 Sigma B9645-500G
MnCl2 Sigma-Aldrich M7634-100G
ZnSO4 Sigma Z0251-100G
Na2MoO4 Aldrich 737-860-5G
NaCl2 Fisher Scientific S271-1
CoCl Sigma-Aldrich 232696-5G
FeEDTA Sigma E6760-100G
“Stericup & Steritop” bottle  Milipore Corporation SCGVU02RE Micronutrient container
www.milipore.com
For root wash buffer preparation
EDTA Acros Organics A0305456
Tris Fisher Scientific BP154-1
For hydroponic setup
Autoclavable foam tube plug Jaece Industries Inc. L800-A Identi-Plugs fit to holes with 2R = 6-13 mm
Foam Board Styrofoam Brand  Dow ESR-2142 Thickness is 1/2 inches
Cork borer Humboldt H-9662 Cork Borer Sets with Handles, , Plated Brass Set of 6, 3/16" to 1/2" OD Size
Air pump Aqua Culture MK-1504
Air pump Marketed by Wal-mart Stores, Inc.
Airline tubing and aquarium bubble stones Aqua Culture Tubing: 928/25-S
Airline tubing and aquarium bubble stones Marketed by Wal-mart Stores, Inc. Stone: ASC-1
Other
Ethanol Fisher Scientific A995-4 Reagent Alcohol
Cadmium Chloride (CdCl2) Sigma-Aldrich 10108-64-2

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