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JoVE Journal Bioengineering
Two Methods for Decellularization of Plant Tissues for Tissue Engineering Applications
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Two Methods for Decellularization of Plant Tissues for Tissue Engineering Applications

Article DOI: 10.3791/57586-v • 05:20 min • May 31st, 2018
May 31st, 2018 •
1, 2, 4, 4, 1, 2,3
1Department of Surgery, University of Wisconsin-Madison, 2Department of Orthopedics and Rehabilitation, University of Wisconsin School of Medicine and Public Health, 3Department of Biomedical Engineering, University of Wisconsin College of Engineering, 4Department of Biomedical Engineering, Worcester Polytechnic Institute

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Summary
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Here we present, and contrast two protocols used to decellularize plant tissues: a detergent-based approach and a detergent-free approach. Both methods leave behind the extracellular matrix of the plant tissues used, which can then be utilized as scaffolds for tissue engineering applications.

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Decellularization Plant Tissues Tissue Engineering Biomaterials Scaffold Fluid Transfer Ultra-structures Manufacturing Specialized Equipment F.hispida Leaves Fresh Samples Frozen Samples Deionized Water Biopsy Punch Wash SDS Solution Plastic Dish Incubation Evaporation Non-ionic Surfactant Bleach
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