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3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol

Article DOI: 10.3791/58911-v • 10:14 min • May 12th, 2019
May 12th, 2019 •
1, 1, 2, 1
1Graduate School of Medicine and Faculty of Medicine, Kyoto University, 2Graduate School of Informatics, Kyoto University

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This article introduces an experimental protocol using 3D scanning technology bridging two spatial scales: the macroscopic spatial scale of whole-brain anatomy imaged by MRI at >100 μm and the microscopic spatial scale of neuronal distributions using immunohistochemistry staining and a multielectrode array system and other methods (~10 μm).

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3D Scanning Technology Microcircuits Macroscale Brain Images 3D Embedding Protocol Neuroanatomical Research Overlapping Performance 3D Neuro Protocol Spatial Scales Human MRIs Post-mortem Brain MRI Contrast Patterns Water Sensitivity Mouse Preparation MRI Acquisitions Equipment Preparations Skull Removal Cutting Solution Gas Bomb
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