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Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
DOI:

09:22 min

February 28, 2021

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Chapters

  • 00:05Introduction
  • 01:03Design and Construction of BRCA1 Targeting gRNAs
  • 03:17Creation of BRCA1 Variants using CRISPR-mediated Base Editing Tools
  • 04:15Sample Preparation for Illumina Next-Generation Sequencing (NGS)
  • 07:01Results: Functional Study of BRCA1 using CRISPR-Cas9 Systems
  • 08:34Conclusion

Summary

Automatic Translation

People with BRCA1 mutations have a higher risk of developing cancer, which warrants accurate evaluation of the function of BRCA1 variants. Herein, we described a protocol for functional assessment of BRCA1 variants using CRISPR-mediated cytosine base editors that enable targeted C:G to T:A conversion in living cells.

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