Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Cancer Research

A subscription to JoVE is required to view this content.
You will only be able to see the first 20 seconds.

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
 

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Article doi: 10.3791/61557
February 28th, 2021

Summary February 28th, 2021

People with BRCA1 mutations have a higher risk of developing cancer, which warrants accurate evaluation of the function of BRCA1 variants. Herein, we described a protocol for functional assessment of BRCA1 variants using CRISPR-mediated cytosine base editors that enable targeted C:G to T:A conversion in living cells.

Transcript

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter