使用 CRISPR 介质基础编辑器对 BRCA1 变体进行功能评估
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February 28, 2021
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Summary
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BRCA1突变患者患癌症的风险较高，这值得准确评估BRCA1变异的功能。在此，我们描述了使用CRISPR介质细胞素基础编辑器对BRCA1变种进行功能评估的协议，该基编辑器支持活细胞中目标C:G到T:A的转换。
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Cite this Article
See, J., Shin, H. R., Jang, G., Kweon, J., Kim, Y. Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors. J. Vis. Exp. (168), e61557, doi:10.3791/61557 (2021).
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