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ヒト胚性幹細胞のミトコンドリア複合体活性の状態調査
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JoVE Journal
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Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
ヒト胚性幹細胞のミトコンドリア複合体活性の状態調査
DOI:
10.3791/724-v
•
12:42 min
•
June 17, 2008
•
Ivan Khvorostov
,
Jin Zhang
,
Michael Teitell
1
David Geffen School of Medicine
,
University of California, Los Angeles
Chapters
00:03
hESCs and Mitochondrial Complex Activity
00:44
Introduction
01:41
Mitochondrial Isolation
04:08
Solubilizing the Crude Mitochondrial Pellet
05:42
Gel and Sample Preparation and Electrophoresis Conditions
09:47
In-Gel Activity Assays
12:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
このビデオでは、我々は、ヒト胚性幹細胞のミトコンドリアの呼吸鎖複合体は、ゲルの活性アッセイに使用して分析できる方法を紹介します。
Tags
Mitochondrial Complex Activity
Human Embryonic Stem Cells
Cellular Metabolism
Homeostasis
Cell Signaling Network
Programmed Cell Death
ATP Production
Cytoplasmic Redox State
Ca2+ Balance
Fatty Acid Catabolism
Heme Synthesis
Nucleotide Synthesis
Steroid Hormone Synthesis
Amino Acid Synthesis
Iron-sulfur Cluster Assembly
Heat Production
Mitochondrial Genome Mutations
Aging Correlation
Cancer Development Correlation
Stem Cell Viability
Stem Cell Proliferation
Stem Cell Differentiation Capacity
Energy Status Of Stem Cells
Mitochondrial Properties Of Human Embryonic Stem Cells (hESCs)
Precursor Progeny Differentiation
Measurement Of Mitochondrial Respiratory Complexes
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