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Journal
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Biology
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Nuclear Transfer into Mouse Oocytes
JoVE Journal
Biology
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JoVE Journal
Biology
Nuclear Transfer into Mouse Oocytes
Nuclear Transfer into Mouse Oocytes
DOI:
10.3791/116-v
•
14:17 min
•
November 30, 2006
•
Dieter Egli
,
Kevin Eggan
1
Department of Molecular and Cell Biology
,
Harvard
Chapters
00:00
Introduction
00:17
Preparation of dishes
00:18
Dish for oocyte incubation KSOM
01:08
Dish for oocyte isolation HCZB and HCZB/hyaluronidase
02:23
Dish for enucleation and nuclear transfer HCZB/cytocholazin
03:30
Preparation of pipettes
03:31
Holding pipette
04:53
Injection pipette
06:30
Mouth pipette for oocyte isolation
07:07
Oocyte isolation
09:43
Nuclear transfer
09:58
Enucleation
10:31
Collection of donor cells
11:05
Transfer
11:50
Discussion
Summary
Automatic Translation
English (Original)
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Automatic Translation
This movie and the protocol are intended to help learning nuclear transfer.
Tags
Nuclear Transfer
Mouse Oocytes
Developmental Potential
Epigenetic Processes
Genetic Processes
Regenerative Medicine
Embryonic Stem Cells
Cloned Preimplantation Stage Embryos
Stem Cells
In Vitro Study
Nuclear Reprogramming
Prof. Yanagimachi
WAKAYAMA Et Al. 1998
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