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JoVE Journal
Biology
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Engineering Cell-permeable Protein
 

Engineering Cell-permeable Protein

Article DOI: 10.3791/1627-v 21:09 min December 28th, 2009
December 28th, 2009

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Summary

Protein transduction enables the direct delivery of biologically active proteins into cells. In contrast to conventional methods such as DNA transfection or viral transduction this non-invasive paradigm allows highly efficient cellular manipulation in a titratable manner circumventing cellular toxicity and the risk of oncogenic transformation by permanent genetic modification.

Tags

Protein Transduction Cell-permeable Protein Cell Penetrating Peptides Protein Transduction Domains TAT-CPP Nuclear Localization Signal Engineering DNA-modifying Enzyme Cre Site-specific Recombinase LoxP Sites PSESAME Vector System
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