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Two-Photon-Based Photoactivation in Live Zebrafish Embryos
JoVE Journal
Biology
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JoVE Journal Biology
Two-Photon-Based Photoactivation in Live Zebrafish Embryos
DOI:

09:10 min

December 24, 2010

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Chapters

  • 00:05Title
  • 01:03Introduction
  • 01:38Injection of Caged Fluorescein
  • 03:15Mounting of Injected Embryos
  • 04:17Photoactivation
  • 05:59Detection of Uncaged Fluorescein
  • 08:02Tracing the Lineage of Neural Progenitors Using Photoactivation
  • 08:45Conclusion

Summary

Automatic Translation

Multiphoton microscopy allows control of low energy photons with deep optical penetration and reduced phototoxicity. We describe the use of this technology for live cell labeling in zebrafish embryos. This protocol can be readily adapted for photo-induction of various light-responsive molecules.

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