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Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
DOI:

11:21 min

October 06, 2010

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Chapters

  • 00:00Title
  • 00:54Introduction
  • 01:18Static Co-culture Biofilm Model
  • 03:41Flow Cell Co-culture Biofilm Model
  • 08:36Representative Results
  • 10:52Conclusion

Summary

Automatic Translation

This paper describes different methods of growing Pseudomonas aeruginosa biofilms on cultured human airway epithelial cells. These protocols can be adapted to study different aspects of biofilm formation, including visualization of the biofilm, staining of the biofilm, measuring the colony forming units (CFU) of the biofilm, and studying biofilm cytotoxicity.

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