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Biology

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Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney
 

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney

Article DOI: 10.3791/2555-v 08:07 min April 22nd, 2011
April 22nd, 2011

Chapters

Summary

In this report we describe a method for the isolation and culture of the progenitor cell niche from the embryonic mouse kidney that can be used to study signaling pathways regulating stem/progenitor cells of the developing kidney. These cultured cells are highly accessible to small molecule and recombinant protein treatment, and importantly also to viral transduction, which allows efficient manipulation of candidate pathways.

Tags

Isolation Culture Cells Nephrogenic Zone Embryonic Mouse Kidney Epithelial-mesenchymal Interaction Organogenesis Congenital Kidney Disease Embryonic Stem Cells Regenerative Medicine Genetic Studies Pathways Catalog Of Gene Transcription Organ Culture Candidate Proteins SiRNA Morpholinos Signaling Stem/progenitor Cell Differentiation Renewal
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