Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content.

Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex
 

Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex

Article DOI: 10.3791/4328-v 10:45 min December 19th, 2012
December 19th, 2012

Chapters

Summary

A procedure is described for manipulating the activity of cerebral cortical pyramidal neurons optogenetically while the electroencephalogram, electromyogram, and cerebral lactate concentration are monitored. Experimental recordings are performed on cable-tethered mice while they undergo spontaneous sleep/wake cycles. Optogenetic equipment is assembled in our laboratory; recording equipment is commercially available.

Tags

Simultaneous Electroencephalography Real-time Measurement Lactate Concentration Optogenetic Manipulation Neuronal Activity Rodent Cerebral Cortex Glucose Utilization Non Rapid Eye Movement Sleep Cerebral Metabolism Slow Waves Electroencephalogram Depolarized/up State Hyperpolarized/down State Action Potentials
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter