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JoVE Journal
Biology

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Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination
 

Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination

Article DOI: 10.3791/4426-v 12:01 min December 31st, 2012
December 31st, 2012

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Summary

We describe a protocol using chamber slides and media to immobilize plant cotyledons for confocal imaging of the epidermis over several days of development, documenting stomatal differentiation. Fluorophore-tagged proteins can be tracked dynamically by expression and subcellular localization, increasing understanding of their possible roles during cell division and cell-type differentiation.

Tags

Confocal Time Lapse Imaging Arabidopsis Cotyledon Cellular Behavior Tissue Patterning In Vivo Dynamics Plant Development Long-term Live Imaging Cell Division Differentiation Events Arabidopsis Epidermis Signaling Cell Fate Stomata Asymmetric Division Cell Differentiation Steps
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